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The map label for this gene is proS

Identifier: 15836031

GI number: 15836031

Start: 579979

End: 581685

Strand: Direct

Name: proS

Synonym: CPj0500

Alternate gene names: 15836031

Gene position: 579979-581685 (Clockwise)

Preceding gene: 15836027

Following gene: 15836032

Centisome position: 47.28

GC content: 45.4

Gene sequence:

>1707_bases
ATGAAAACGTCTCAACTCTTTTATAAGACTTCAAAAAATGCAAATAAAAGCGCTGCTGTGCTCTCAAACGAGCTCCTAGA
AAAGGCAGGATACCTATTTAAAGTAAGTAAAGGAGTCTATACCTATACACCCCTGTTATGGCGCGTGGTCTCCAAGATGA
TGAACATCATTAGAGAGGAACTTAATGCGATTGGAGGTCAAGAACTTCTACTCCCACTTCTCCACAATGCTGAACTTTGG
CAACATACAGGGAGATGGGAGGCATTTACTTCGGAAGGACTGCTCTACACTCTCAAAGACCGCGAAGGAAAATCTCATTG
CCTAGCTCCTACACATGAAGAGGTCATCTGCTCTTTTGTTGCACAATGGCTCTCCTCAAAAAGACAACTTCCTCTCCACC
TTTACCAAATTGCTACAAAATTCCGAGACGAGATTCGCCCTCGATTCGGTCTCATTCGCTCTCGAGAGCTCCTTATGGAA
GACAGCTATACCTTCTCAGACTCTCCCGAACAAATGAACGAGCAATATGAAAAACTCCGCTCTGCGTATAGTAAGATCTT
TGATCGTCTCGGTCTTGCCTATGTCATCGTTACAGCTGATGGAGGGAAAATCGGCAAAGGAAAGTCTGAGGAATTTCAGG
TCCTTTGCTCTCTAGGCGAGGACACGATCTGCGTCAGCGGTTCCTATGGAGCTAATATTGAGGCTGCTGTCTCCATTCCT
CCACAGCATGCCTACGATCGCGAGTTTCTTCCCGTCGAAGAAGTGGCCACCCCTGGGATTACAACAATAGAAGCTCTAGC
AAACTTCTTCTCTATCCCCTTACATAAAATTTTAAAAACCCTTGTCGTAAAACTCTCCTACTCAAATGAAGAAAAATTCA
TTGCCATTGGAATGAGAGGAGATCGGCAAGTCAACCTAGTGAAGGTCGCTTCCAAACTGAATGCCGATGATATTGCTCTA
GCTTCTGATGAAGAAATCGAACGCGTTCTAGGCACAGAAAAAGGATTCATCGGTCCCCTAAACTGTCCCATAGACTTTTT
CGCAGACGAAACAACGTCCCCAATGACGAACTTTGTTTGTGCGGGCAATGCTAAAGATAAGCACTACGTAAATGTAAACT
GGGATCGCGACCTCCTCCCCCCCCAATACGGTGACTTTCTACTCGCTGAAGAGGGAGACACATGTCCTGAAAATCCTGGC
CATCCTTACCGCATTTATCAAGGCATAGAAGTTGCTCATATTTTCAATCTCGGGACACGCTATACCGATAGTTTTGAGGT
AAACTTCCTAGATGAACACGGGCAAACCCAGCAGTGCTGGATGGGGACCTACGGCATTGGAGTCGGAAGAACATTAGCCG
CTTGTGTAGAACAGCTTCCCGACGACCGTGGTATTGTTTGGCCAAAAGCACTCGCTCCCTTCTCTATCACTATCGCCTTT
AACGGAGGAGACACTGTATCTCAAGAGCTTGCGGAAACTATTTATCATGAGCTACAAAGTCAAGGCTATGAGCCCCTTCT
TGATGATCGAGATGAAAGACTCGGATTTAAACTTAAAGACAGTGACCTTATCGGCATTCCTTATAAGCTTATTTTAGGAA
AGTCCTACCAATCTTCGGGAATATTCGAAATTGAATCCCGATCTGGAGAAAAGTATACAGTCTCCCCGGAGGCCTTCCCT
ACTTGGTGTCAGAATCACTTAGCCTAG

Upstream 100 bases:

>100_bases
ATCTACTAGCTTAAAAGACTCTCGATATAAAATCGCAATAGCCTGTAATTTTTTCTATAGACTCTTGACGATCAATCACT
GAAGATAACAGCCCTACTCT

Downstream 100 bases:

>100_bases
CTCTTTGATCGCTGCCCTCCTCGTAAAAAAGTTAGCATTCATTCAATTCGAGTGCTAAATTCTCTTGACCTTCTCGGGTT
CTTTTCCTATAATGCTCTCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKTSQLFYKTSKNANKSAAVLSNELLEKAGYLFKVSKGVYTYTPLLWRVVSKMMNIIREELNAIGGQELLLPLLHNAELW
QHTGRWEAFTSEGLLYTLKDREGKSHCLAPTHEEVICSFVAQWLSSKRQLPLHLYQIATKFRDEIRPRFGLIRSRELLME
DSYTFSDSPEQMNEQYEKLRSAYSKIFDRLGLAYVIVTADGGKIGKGKSEEFQVLCSLGEDTICVSGSYGANIEAAVSIP
PQHAYDREFLPVEEVATPGITTIEALANFFSIPLHKILKTLVVKLSYSNEEKFIAIGMRGDRQVNLVKVASKLNADDIAL
ASDEEIERVLGTEKGFIGPLNCPIDFFADETTSPMTNFVCAGNAKDKHYVNVNWDRDLLPPQYGDFLLAEEGDTCPENPG
HPYRIYQGIEVAHIFNLGTRYTDSFEVNFLDEHGQTQQCWMGTYGIGVGRTLAACVEQLPDDRGIVWPKALAPFSITIAF
NGGDTVSQELAETIYHELQSQGYEPLLDDRDERLGFKLKDSDLIGIPYKLILGKSYQSSGIFEIESRSGEKYTVSPEAFP
TWCQNHLA

Sequences:

>Translated_568_residues
MKTSQLFYKTSKNANKSAAVLSNELLEKAGYLFKVSKGVYTYTPLLWRVVSKMMNIIREELNAIGGQELLLPLLHNAELW
QHTGRWEAFTSEGLLYTLKDREGKSHCLAPTHEEVICSFVAQWLSSKRQLPLHLYQIATKFRDEIRPRFGLIRSRELLME
DSYTFSDSPEQMNEQYEKLRSAYSKIFDRLGLAYVIVTADGGKIGKGKSEEFQVLCSLGEDTICVSGSYGANIEAAVSIP
PQHAYDREFLPVEEVATPGITTIEALANFFSIPLHKILKTLVVKLSYSNEEKFIAIGMRGDRQVNLVKVASKLNADDIAL
ASDEEIERVLGTEKGFIGPLNCPIDFFADETTSPMTNFVCAGNAKDKHYVNVNWDRDLLPPQYGDFLLAEEGDTCPENPG
HPYRIYQGIEVAHIFNLGTRYTDSFEVNFLDEHGQTQQCWMGTYGIGVGRTLAACVEQLPDDRGIVWPKALAPFSITIAF
NGGDTVSQELAETIYHELQSQGYEPLLDDRDERLGFKLKDSDLIGIPYKLILGKSYQSSGIFEIESRSGEKYTVSPEAFP
TWCQNHLA
>Mature_568_residues
MKTSQLFYKTSKNANKSAAVLSNELLEKAGYLFKVSKGVYTYTPLLWRVVSKMMNIIREELNAIGGQELLLPLLHNAELW
QHTGRWEAFTSEGLLYTLKDREGKSHCLAPTHEEVICSFVAQWLSSKRQLPLHLYQIATKFRDEIRPRFGLIRSRELLME
DSYTFSDSPEQMNEQYEKLRSAYSKIFDRLGLAYVIVTADGGKIGKGKSEEFQVLCSLGEDTICVSGSYGANIEAAVSIP
PQHAYDREFLPVEEVATPGITTIEALANFFSIPLHKILKTLVVKLSYSNEEKFIAIGMRGDRQVNLVKVASKLNADDIAL
ASDEEIERVLGTEKGFIGPLNCPIDFFADETTSPMTNFVCAGNAKDKHYVNVNWDRDLLPPQYGDFLLAEEGDTCPENPG
HPYRIYQGIEVAHIFNLGTRYTDSFEVNFLDEHGQTQQCWMGTYGIGVGRTLAACVEQLPDDRGIVWPKALAPFSITIAF
NGGDTVSQELAETIYHELQSQGYEPLLDDRDERLGFKLKDSDLIGIPYKLILGKSYQSSGIFEIESRSGEKYTVSPEAFP
TWCQNHLA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=216, Percent_Identity=45.8333333333333, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1786392, Length=561, Percent_Identity=43.3155080213904, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI115532348, Length=218, Percent_Identity=36.2385321100917, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320931, Length=564, Percent_Identity=28.5460992907801, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24656200, Length=241, Percent_Identity=36.5145228215768, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63819; Mature: 63819

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSQLFYKTSKNANKSAAVLSNELLEKAGYLFKVSKGVYTYTPLLWRVVSKMMNIIREE
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIGGQELLLPLLHNAELWQHTGRWEAFTSEGLLYTLKDREGKSHCLAPTHEEVICSFV
HHHCCCHHHHHHHHCCCHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
AQWLSSKRQLPLHLYQIATKFRDEIRPRFGLIRSRELLMEDSYTFSDSPEQMNEQYEKLR
HHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SAYSKIFDRLGLAYVIVTADGGKIGKGKSEEFQVLCSLGEDTICVSGSYGANIEAAVSIP
HHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCEEEEEECC
PQHAYDREFLPVEEVATPGITTIEALANFFSIPLHKILKTLVVKLSYSNEEKFIAIGMRG
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCCEEEEECCC
DRQVNLVKVASKLNADDIALASDEEIERVLGTEKGFIGPLNCPIDFFADETTSPMTNFVC
CCCCHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHEEE
AGNAKDKHYVNVNWDRDLLPPQYGDFLLAEEGDTCPENPGHPYRIYQGIEVAHIFNLGTR
ECCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCC
YTDSFEVNFLDEHGQTQQCWMGTYGIGVGRTLAACVEQLPDDRGIVWPKALAPFSITIAF
CCCCEEEEEECCCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEE
NGGDTVSQELAETIYHELQSQGYEPLLDDRDERLGFKLKDSDLIGIPYKLILGKSYQSSG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEECCCCEECCCCEEEECCCCCCCC
IFEIESRSGEKYTVSPEAFPTWCQNHLA
CEEEECCCCCEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MKTSQLFYKTSKNANKSAAVLSNELLEKAGYLFKVSKGVYTYTPLLWRVVSKMMNIIREE
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIGGQELLLPLLHNAELWQHTGRWEAFTSEGLLYTLKDREGKSHCLAPTHEEVICSFV
HHHCCCHHHHHHHHCCCHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
AQWLSSKRQLPLHLYQIATKFRDEIRPRFGLIRSRELLMEDSYTFSDSPEQMNEQYEKLR
HHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SAYSKIFDRLGLAYVIVTADGGKIGKGKSEEFQVLCSLGEDTICVSGSYGANIEAAVSIP
HHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCEEEEEECC
PQHAYDREFLPVEEVATPGITTIEALANFFSIPLHKILKTLVVKLSYSNEEKFIAIGMRG
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCCEEEEECCC
DRQVNLVKVASKLNADDIALASDEEIERVLGTEKGFIGPLNCPIDFFADETTSPMTNFVC
CCCCHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHEEE
AGNAKDKHYVNVNWDRDLLPPQYGDFLLAEEGDTCPENPGHPYRIYQGIEVAHIFNLGTR
ECCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCC
YTDSFEVNFLDEHGQTQQCWMGTYGIGVGRTLAACVEQLPDDRGIVWPKALAPFSITIAF
CCCCEEEEEECCCCCCCHHEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEE
NGGDTVSQELAETIYHELQSQGYEPLLDDRDERLGFKLKDSDLIGIPYKLILGKSYQSSG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEECCCCEECCCCEEEECCCCCCCC
IFEIESRSGEKYTVSPEAFPTWCQNHLA
CEEEECCCCCEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]